TISSUE-CULTURE AND PROTOPLAST ISOLATION IN ASPARAGUS DENSIFLORUS L.
نویسندگان
چکیده
منابع مشابه
Production of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC
To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...
متن کاملProduction of sarsasapogenin from tissue culture of Asparagus racemosus and its quantification by HPTLC
To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...
متن کاملIsolation of Adipose Tissue Stem Cells with Organ Culture Method
Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...
متن کاملObservations and Suggestions for Improving Somatic Hybridization by Plant Protoplast Isolation, Fusion, and Culture
De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...
متن کاملProtoplast isolation and transient gene expression in switchgrass, Panicum virgatum L.
Transient assay systems using protoplasts have been utilized in several plant species and are a powerful tool for rapid functional gene analysis and biochemical manipulations. A protoplast system has not been used in switchgrass (Panicum virgatum L.), even though it is a bioenergy crop that has received considerable attention. Here we report the first protocol to isolate large numbers of viable...
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ژورنال
عنوان ژورنال: HortScience
سال: 1993
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.28.4.259g